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KMID : 1001920120510060323
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Journal of Korean Neurosurgical Society
2012 Volume.51 No. 6 p.323 ~ p.327
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The Change of Bone Metabolism in Ovariectomized Rats : Analyses of MicroCT Scan and Biochemical Markers of Bone Turnover
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Yoon Kyung-Hyuk
Cho Dae-Chul
Yu Song-Hee
Kim Kyung-Tae
Jeon Young-Hoon
Sung Joo-Kyung
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Abstract
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Objective: The purpose of this study was to verify the appropriateness of ovariectomized rats as the osteoporosis animal model.
Methods: Twelve female Sprague-Dawley rats underwent a sham operation (the sham group) or bilateral ovariectomy [the ovariectomy (OVX) group]. Eight weeks after operations, serum biochemical markers of bone turnover were analyzed; osteocalcin and alkaline phosphatase, which are sensitive biochemical markers of bone formation, and C-terminal telopeptide fragment of type I collagen C-terminus (CTX), which is a sensitive biochemical marker of bone resorption. Bone histomorphometric parameters and microarchitectural properties of 4th lumbar vertebrae were determined by micro-computed tomographic (CT) scan.
Results: The OVX group showed on average 75.4% higher osteocalcin and 72.5% higher CTX levels than the sham group, indicating increased bone turnover. Micro-CT analysis showed significantly lower bone mineral density (BMD) (p=0.005) and cortical BMD (p=0.021) in the OVX group. Furthermore, the OVX group was found to have a significantly lower trabecular bone volume fraction (p=0.002).
Conclusion: Our results showed that bone turnover was significantly increased and bone mass was significantly decreased 8 weeks after ovariectomy in rats. Thus, we propose that the ovariectomized rat model be considered a reproducible and reliable model of osteoporosis.
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KEYWORD
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Bone loss, Bone turnover, Osteoporosis, Ovariectomy, Rat
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